EXCITE THEM ALL!
Imagine simultaneously imaging a wide range of fluorophores without having to think about selecting the optimum excitation wavelength of your laser. In two-photon microscopy, this is often a complex and sometimes an impossible task when using traditional 100fs excitation lasers.
Transform-limited 100fs solid-state lasers emitting in the IR have a spectrum in the range of 10 – 20nm and as a result, they can only simultaneously excite fluorophores whose excitation spectra falls within this 10-20nm spectrum.
To simultaneously excite a larger variety of fluorophores with a single laser, lasers with broader bandwidths and shorter pulse durations are required.
For example, a laser with 15 fs pulses, such as FYLA´s SCH laser, centered at 1050nm, delivers 15fs pulses and a bandwidth of 200nm. All green and red fluorophores within this bandwidth (which extends across 900 to 1200nm) can be excited simultaneously by such laser.